Zoo animals as reservoirs of gram-negative bacteria harboring integrons and antimicrobial resistance genes.
نویسندگان
چکیده
A total of 232 isolates of gram-negative bacteria were recovered from mammals, reptiles, and birds housed at Asa Zoological Park, Hiroshima prefecture, Japan. Forty-nine isolates (21.1%) showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. PCR and DNA sequencing identified class 1 and class 2 integrons and many beta-lactamase-encoding genes, in addition to a novel AmpC beta-lactamase gene, bla(CMY-26). Furthermore, the plasmid-mediated quinolone resistance genes qnr and aac(6')-Ib-cr were also identified.
منابع مشابه
Isolation and molecular characterization of multidrug-resistant Gram-negative bacteria from imported flamingos in Japan
Imported animals, especially those from developing countries, may constitute a potential hazard to native animals and to public health. In this study, a new flock of lesser flamingos imported from Tanzania to Hiroshima Zoological Park were screened for multidrug-resistant Gram-negative bacteria, integrons and antimicrobial resistance genes. Thirty-seven Gram-negative bacterial isolates were obt...
متن کاملLong-term stability of temocillin in elastomeric pumps for outpatient antibiotic therapy in cystic fibrosis patients.
s of the Second ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in Animals, Humans and the Environment, Toronto, 2010. Abstract 87A, p. 13. American Society for Microbiology, Washington, DC, USA. 3 Garcı́a-Fernández A, Fortini D, Veldman K et al. Characterization of plasmids harbouring qnrS1, qnrB2 and qnrB19 genes in Salmonella. J Antimicrob Chemother 200...
متن کاملAsymptomatic intramammary infection with multidrug-resistant gram-negative bacteria in a research dairy farm: incidence and genetic basis of resistance.
Nonpathogenic and opportunistic bacteria are increasingly recognized as reservoirs of antibiotic resistance genes. However, nothing is known about the mechanisms of antibiotic resistance in such bacteria isolated from the udders of healthy animals. In this study, 150 Gram-negative strains isolated from milk samples of healthy dairy cows were screened for the presence of a large pool of antibiot...
متن کاملRapid screening technique for class 1 integrons in Enterobacteriaceae and nonfermenting gram-negative bacteria and its use in molecular epidemiology.
A screening technique for integrons in members of the family Enterobacteriaceae and nonfermenting gram-negative bacteria by real-time PCR is reported. A total of 226 isolates of gram-negative bacteria obtained from a variety of clinical specimens were screened for class 1 integrons by real-time PCR performed on a LightCycler instrument. This technique used a primer pair specific for a 300-bp co...
متن کاملEvidence of extensive interspecies transfer of integron-mediated antimicrobial resistance genes among multidrug-resistant Enterobacteriaceae in a clinical setting.
Multidrug resistance in gram-negative bacteria appears to be primarily the result of the acquisition of resistance genes by horizontal transfer. To what extent horizontal transfer may be responsible for the emergence of multidrug resistance in a clinical setting, however, has rarely been investigated. Therefore, the integron contents of isolates collected during a nosocomial outbreak of genotyp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 73 20 شماره
صفحات -
تاریخ انتشار 2007